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文献精选 > 近年高分"单细胞测序"研究（IF≥30，近5年）

Using single-cell sequencing technology to detect circulating tumor cells in solid tumors. ​

文献信息 ​

DOI10.1186/s12943-021-01392-w
PMID34412644
期刊Molecular cancer
影响因子33.9
JCR 分区Q1
发表年份2021
被引次数55
关键词循环肿瘤细胞, 研究进展, 单细胞测序, 肿瘤异质性
文献类型Journal Article, Research Support, Non-U.S. Gov't, Review
ISSN1476-4598
页码104
期号20(1)
作者Jiasheng Xu, Kaili Liao, Xi Yang, Chengfeng Wu, Wei Wu

一句话小结 ​

本文探讨了循环肿瘤细胞的特性及其在肿瘤研究中的重要性，指出通过单细胞测序技术可以有效识别和分析循环肿瘤细胞，克服肿瘤组织异质性带来的挑战，从而为肿瘤生物过程的理解和临床应用提供了新的视角与方法。这一研究为肿瘤的分型、转移监测及治疗策略的优化奠定了基础。

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循环肿瘤细胞 · 研究进展 · 单细胞测序 · 肿瘤异质性

摘要 ​

循环肿瘤细胞是具有高活力和高转移潜力的肿瘤细胞，它们从原发实体肿瘤或转移灶侵入并释放到外周血中。由于肿瘤的异质性，高通量测序分析肿瘤组织时很难找到低丰度肿瘤干细胞的基因组特征。循环肿瘤细胞的单细胞测序通过比较循环肿瘤细胞、原发肿瘤、转移肿瘤及患者外周血中的转移淋巴结之间单细胞基因组、转录组和表观遗传组的差异，避免了肿瘤异质性的干扰，为理解肿瘤的生物过程提供了新的视角。本文描述了循环肿瘤细胞的识别、生物特征及单细胞全基因组变异，并总结了单细胞测序技术在肿瘤分型、转移分析、进展检测和辅助治疗中的应用。

英文摘要 ​

Circulating tumor cells are tumor cells with high vitality and high metastatic potential that invade and shed into the peripheral blood from primary solid tumors or metastatic foci. Due to the heterogeneity of tumors, it is difficult for high-throughput sequencing analysis of tumor tissues to find the genomic characteristics of low-abundance tumor stem cells. Single-cell sequencing of circulating tumor cells avoids interference from tumor heterogeneity by comparing the differences between single-cell genomes, transcriptomes, and epigenetic groups among circulating tumor cells, primary and metastatic tumors, and metastatic lymph nodes in patients' peripheral blood, providing a new perspective for understanding the biological process of tumors. This article describes the identification, biological characteristics, and single-cell genome-wide variation in circulating tumor cells and summarizes the application of single-cell sequencing technology to tumor typing, metastasis analysis, progression detection, and adjuvant therapy.

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主要研究问题 ​

  1. 单细胞测序技术如何提高对循环肿瘤细胞的检测灵敏度和特异性？
  2. 在循环肿瘤细胞的单细胞基因组分析中，如何处理细胞间的异质性问题？
  3. 除了肿瘤分型，单细胞测序技术在肿瘤转移分析中的其他潜在应用是什么？
  4. 循环肿瘤细胞的生物特征如何影响其在不同类型固体肿瘤中的检测和分析？
  5. 单细胞测序技术在肿瘤进展检测和辅助治疗中的具体应用案例有哪些？

核心洞察 ​

研究背景和目的 ​

循环肿瘤细胞（CTCs）是从原发性或转移性肿瘤中脱落到外周血液中的活性肿瘤细胞，具有高度的转移潜力。由于肿瘤的异质性，传统的高通量测序方法难以识别低丰度肿瘤干细胞的基因特征。本文旨在探讨单细胞测序技术在检测实体肿瘤中CTCs的应用，以揭示肿瘤的生物学过程。

主要方法/材料/实验设计 ​

研究采用单细胞测序技术，比较外周血中CTCs与原发性和转移性肿瘤及转移淋巴结的基因组、转录组和表观遗传组之间的差异，避免肿瘤异质性对结果的干扰。

Mermaid diagram

关键结果和发现 ​

  1. CTCs在外周血中的数量和表型与原发肿瘤的进展密切相关，CTCs的出现可以作为监测肿瘤进展的重要指标。
  2. 单细胞测序揭示了CTCs的高度异质性，发现不同CTCs之间存在显著的基因突变和拷贝数变化（CNV）。
  3. CTCs的基因组特征与原发肿瘤和转移灶之间的关系复杂，CTCs能够反映肿瘤的转移和耐药机制。

主要结论/意义/创新性 ​

单细胞测序技术为理解肿瘤的发生与发展提供了新的视角，能够揭示CTCs的遗传异质性和转移机制。该技术在肿瘤分型、转移分析、进展检测和辅助治疗中的应用具有重要意义，尤其是在个体化医疗方面。

研究局限性和未来方向 ​

研究面临的主要挑战包括CTCs的低丰度和样本多样性，这可能影响测序结果的准确性。此外，单细胞测序技术仍需优化以提高覆盖率和均匀性。未来的研究应聚焦于改进单细胞测序的技术手段，结合多组学分析，进一步探索CTCs在肿瘤异质性、进展和耐药性中的作用。

参考文献 ​

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引用本文的文献 ​

  1. Circulating tumor cells: biology and clinical significance. - Danfeng Lin;Lesang Shen;Meng Luo;Kun Zhang;Jinfan Li;Qi Yang;Fangfang Zhu;Dan Zhou;Shu Zheng;Yiding Chen;Jiaojiao Zhou - Signal transduction and targeted therapy (2021)
  2. Single-Cell RNA Sequencing Reveals Multiple Pathways and the Tumor Microenvironment Could Lead to Chemotherapy Resistance in Cervical Cancer. - Meijia Gu;Ti He;Yuncong Yuan;Suling Duan;Xin Li;Chao Shen - Frontiers in oncology (2021)
  3. Single-Cell RNA-Seq Reveals the Promoting Role of Ferroptosis Tendency During Lung Adenocarcinoma EMT Progression. - Jiaxi Yao;Yuchong Zhang;Mengling Li;Zuyu Sun;Tao Liu;Mingfang Zhao;Zhi Li - Frontiers in cell and developmental biology (2021)
  4. Recent advances and application of whole genome amplification in molecular diagnosis and medicine. - Xiaoyu Wang;Yapeng Liu;Hongna Liu;Wenjing Pan;Jie Ren;Xiangming Zheng;Yimin Tan;Zhu Chen;Yan Deng;Nongyue He;Hui Chen;Song Li - MedComm (2022)
  5. Correction to: Using single-cell sequencing technology to detect circulating tumor cells in solid tumors. - Jiasheng Xu;Kaili Liao;Xi Yang;Chengfeng Wu;Wei Wu - Molecular cancer (2022)
  6. Recent advances in targeted drug delivery systems for resistant colorectal cancer. - Masoumeh Sharifi-Azad;Marziyeh Fathi;William C Cho;Abolfazl Barzegari;Hamed Dadashi;Mehdi Dadashpour;Rana Jahanban-Esfahlan - Cancer cell international (2022)
  7. Clonal barcoding with qPCR detection enables live cell functional analyses for cancer research. - Qiuchen Guo;Milos Spasic;Adam G Maynard;Gregory J Goreczny;Amanuel Bizuayehu;Jessica F Olive;Peter van Galen;Sandra S McAllister - Nature communications (2022)
  8. A narrative review of circulating tumor cells clusters: A key morphology of cancer cells in circulation promote hematogenous metastasis. - Qiong Chen;Jueyao Zou;Yong He;Yanhong Pan;Gejun Yang;Han Zhao;Ying Huang;Yang Zhao;Aiyun Wang;Wenxing Chen;Yin Lu - Frontiers in oncology (2022)
  9. ZEB1-activated Notch1 promotes circulating tumor cell migration and invasion in lung squamous cell carcinoma. - Yong Gao;Xinyuan Cheng;Mingfeng Han - Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico (2023)
  10. Spatial RNA sequencing methods show high resolution of single cell in cancer metastasis and the formation of tumor microenvironment. - Yue Zheng;Xiaofeng Yang - Bioscience reports (2023)

... (45 更多 篇文献)

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